Design of high-affinity S100-target hybrid proteins

Unique S100 target protein interactions.

Three-dimensional structures of S100B, S100A1, S100A6 and S100A11 have shown that calcium binding to these proteins results in a conformational change allowing them to interact with many biological targets. The structures of some S100 proteins in the presence of peptide targets from Ndr kinase, p53, CapZ, annexins A1 and A2 and the Siah-1 Interacting Protein indicate there are at least three mo...

Spectroscopic characterization of a high-affinity calmodulin-target peptide hybrid molecule.

We describe the properties of a hybrid protein comprising the full length of the Xenopus laevis calmodulin sequence, followed by a pentapeptide linker (GGGGS), and residues 3-26 of M13, the calmodulin binding region of skeletal muscle myosin light chain kinase. The properties of the hybrid protein are compared with those of the complex formed between Drosophila calmodulin and a peptide correspo...

affinity based target deconvolution of safranal

Role of S100 Proteins in Colorectal Carcinogenesis

The family of S100 proteins represents 25 relatively small (9-13 kD) calcium binding proteins. These proteins possess a broad spectrum of important intracellular and extracellular functions. Colorectal cancer is the third most common cancer in men (after lung and prostate cancer) and the second most frequent cancer in women (after breast cancer) worldwide. S100 proteins are involved in the colo...

Discovering high-affinity ligands for proteins.

Drug discovery is a difficult and time-consuming process that involves the identification of potent therapeutic | TECH VI EW I agents with good bioavailability, metabolic stability, and low toxicity. An important first step in this process is the identification of molecules that bind with high affinity and selectivity to the biological target. High-affinity ligands might be discovered by screen...